A CDC42 homologue in Claviceps purpurea is involved in vegetative differentiation and is essential for pathogenicity.

A Laboratory Guide to the Identification of Claviceps purpurea and Claviceps africana in Grass and Sorghum Seed Samples

Production of peptide ergot alkaloids in submerged culture by three isolates of Claviceps purpurea.

Three strains of Claviceps purpurea (Fr.) Tul., isolated from sclerotia grown on rye, produce under submerged conditions ergocryptine and ergotamine, ergocornine and ergosine, and ergocristine, respectively. All of the strains either lacked the ability to produce conidia or formed them sparingly, but they accumulated large quantities of lipids and sterols. The fermentations are typically divide...

BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea.

The gene bcsak1, encoding a mitogen-activated protein kinase (MAPK) of Botrytis cinerea, was cloned and characterized. The protein has high homology to the yeast Hog1 and to corresponding MAPKs from filamentous fungi, but it shows unique functional features. The protein is phosphorylated under osmotic stress, specific fungicides, and oxidative stress mediated by H(2)O(2) and menadione. Northern...

The Epipolythiodiketopiperazine Gene Cluster in Claviceps purpurea: Dysfunctional Cytochrome P450 Enzyme Prevents Formation of the Previously Unknown Clapurines

Claviceps purpurea is an important food contaminant and well known for the production of the toxic ergot alkaloids. Apart from that, little is known about its secondary metabolism and not all toxic substances going along with the food contamination with Claviceps are known yet. We explored the metabolite profile of a gene cluster in C. purpurea with a high homology to gene clusters, which are r...

Effect of Chlorsulfuron, a Potent Inhibitor of Acetohydroxyacid Synthase, on Metabolism of Claviceps purpurea

Chlorsulfuron (CS) is a potent sulfonylurea herbicide inhibiting specifically acetohydroxyacid synthase which catalyzes the first step in the biosynthesis of branched-chain amino acids in plant cells, bacteria and yeast. The peptide portion of some ergot alkaloids contain inter alia branchedchain amino acids. The influence of CS on growth and alkaloid formation in Claviceps was studied. In an e...